Corresponding author: Niamh Eastwood (
Academic editor:
Analysis of multiple marker genes using metabarcoding of environmental DNA (eDNA) can offer information greater than that from sequencing single marker genes, such as responses from across the phylogenetic tree to environmental gradients (
Here, we have designed and optimised a multiplex panel of four marker genes (two regions of 18S rRNA gene, one region of the 16S rRNA gene and one region of the rbcL gene). By combining steps into a single reaction, the labwork required is decreased, reducing cost and time. This multiplex is compared with a widely available commercial microbial (bacterial and fungal) screening panel and individual library preparations of each marker gene.
Niamh Eastwood
1st DNAQUA International Conference (March 9-11, 2021)
University of Birmingham, UK
University of Birmingham, UK