81urn:lsid:arphahub.com:pub:EE60897E-EDEE-5EC8-86EB-7203BECD195DARPHA Conference AbstractsACA2603-3925Pensoft Publishers10.3897/aca.5.e872248722419874Conference AbstractInternational Conference on DNA Barcoding and BiodiversityFirst results on the genetic variation of the Bulgarian populations of Morimus asper funereus (Mulsant, 1862) (Coleoptera, Cerambycidae)KostovaRumyanarumy.kostova@gmail.com1BobevaAneliуa Borisovahttps://orcid.org/0000-0001-8016-34722BekchievRostislav3Sofia University, Faculty of Biology, Sofia, BulgariaSofia University, Faculty of BiologySofiaBulgariabInstitute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences, Sofia, BulgariabInstitute of Biodiversity and Ecosystem Research, Bulgarian Academy of SciencesSofiaBulgariaNational Museum of Natural History, Bulgarian Academy of Sciences, Sofia, BulgariaNational Museum of Natural History, Bulgarian Academy of SciencesSofiaBulgaria
2022170620225e87224FA0E9405-F669-54FF-8A33-99C97D0EA3ADRumyana Kostova, Aneliуa Borisova Bobeva, Rostislav BekchievThis is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
There is an ambiguity about the taxonomic status of Morimus asper funereus (Mulsant, 1862),a saproxylic longhorn beetle, widespread in Bulgaria and other Balkan countries. It is a protected species under the Habitats Directive (Annex II) and under the Bulgarian Biodiversity Act. A recent molecular study, based on the cytochrome C oxidase subunit I (COI) and the second internal transcribed spacer (ITS2) gene sequences, found that all European and Turkish populations of Morimus should be referred to M. asper (Sulzer, 1776) (Solano et al. 2013). The territory of Bulgaria and some other Balkan countries remain as an unstudied gap in that research. Here we present preliminary results on the genetic divergence and diversity of Morimus populations on the Balkans based on COI gene sequences using material from Bulgaria and Albania and the haplotypes’ sequences of Solano et al. (2013) obtained from GenBank. The material collected from Bulgaria аnd two localities in Albania was identified based on morphology characteristics as M. asper funereus (44 samples), M. verecundus bulgaricus Danilevski et all., 2016 (1 sample from the type locality), Morimus orientalis Reitter, 1894 (1 sample from Strandzha Mts.) and Lamia textor (Linnaeus, 1758) (1 sample) used as an outgroup. All obtained sequences were analyzed and haplotype diversity was estimated. The results show that Bulgarian populations of Morimus demonstrate relatively high haplotype diversity in correspondence to the diversity of the other European populations. Also, a phylogeographical hypothesis of the linkage between Bulgarian and other Balkan populations was developed. Four main lineages of divergence were identified. In addition, our results support the assumption that M. verucundus is a tentative subspecies of the morphologically and genetically variable M. asper.
Bulgarian National Science Fund, grand № KP-06-Н21/1-17.12.2018.
Funding program
Bulgarian National Science Fund, grand № KP-06-Н21/1-17.12.2018.
Grant title
Cybertaxonomic approach to phylogenetic studies of model invertebrate genera (Invertebrata, Arachnida, Insecta) clarifying the problems of origin, formation and conservation of the Invertebrate Fauna of the Balkan Peninsula.
ReferencesSolanoE.ManciniE.CiucciP.MasonF.AudisioP.AntoniniG.2013The EU protected taxon Morimus funereus Mulsant, 1862 (Coleoptera: Cerambycidae) and its western Palaearctic allies: systematics and conservation outcomes1468369410.1007/s10592-013-0461-3