ARPHA Conference Abstracts : Conference Abstract
Conference Abstract
Accessing bioactive potential of cave bacterial extracts
expand article infoTelma Veloso, Ana Sofia Reboleira§, Helena Oliveira, Fernando Gonçalves, Sérgio Marques
‡ University of Aveiro, Aveiro, Portugal
§ University of Copenhagen, Copenhagen, Denmark
Open Access


Recently, there has been an increasing demand for new molecules with bioactivity as a result of the increasing number of multi drug resistant bacteria and also of the need to find treatment for various diseases. Thus, to fill this urging need there has been a great investment in developing new techniques to speed up and increase the possibility of finding new molecules with therapeutic potential. Typically, the first stage of drug discovery process relies on in vitro assays and conventional high-throughput LC-MS-MS approaches. This combined with unique extreme environments, as a source of new molecules, has proved to be a good approach to enlarge natural product drug discovery. Considering the present demand for new bioactive molecules, together with the fact that cave microorganisms are already considered an important, yet largely unexplored source, our study aimed at assessing the bioactive potential of cave bacterial extracts, namely its antimicrobial and antitumoral potential. From six soil samples collected in the Cerâmica cave, at the Sicó karst massif, centre of Portugal, a total of 409 isolates were obtained. After a primary screening with the cross-streak method, 91 isolates showed antagonistic activity against four pathogenic agents (Escherichia coli, Pseudomonas aeruginosa, Aeromonas salmonicida and Bacillus aquimaris). Afterwards the secondary screening was carried out with the three isolates that presented higher antimicrobial activity. For the secondary screening, cell-free supernatants (CFS) were tested against eight pathogenic agents (Escherichia coli, Pseudomonas aeruginosa, Aeromonas salmonicida, Bacillus cereus, Enterococcus faecalis, Klebesiella pneumoniae, Salmonella typhimurium and Staphylococcus aureus). The chemical analysis of the CFS is discussed, as well as the results from the in vitro tests with human cell lines to evaluate its effects on cell morphology, viability and proliferation (Suppl. material 1).


bacteria; cave; antimicrobial activity

Presenting author

Telma Veloso

Presented at

24th International Conference on Subterranean Biology


Thanks are due for the financial support to CESAM (UID/AMB/50017 - POCI-01-0145-FEDER-007638), to FCT/MCTES through national funds (PIDDAC), and the co-funding by the FEDER, within the PT2020 Partnership Agreement and Compete 2020. SM is supported by a research grant by FCT (SFRH/BPD/112803/2015). Telma Veloso received financial support by Muxima Bio PT for developing her MSc thesis. ASR is supported by a research grant (15471) from the VILLUM FONDEN.

Hosting institution

University of Aveiro

Supplementary material

Suppl. material 1: Accessing bioactive potential of cave bacterial extracts 
Authors:  Veloso, Telma; Reboleira, Ana Sofia P.S.; Gonçalves, Fernando J. M.; Oliveira, Helena; Marques, Sérgio M.
Data type:  Poster pdf