ARPHA Conference Abstracts : Conference Abstract
Conference Abstract
Body burden of pesticides and shape asymmetry in carabid beetles in different agricultural practices in Mediterranean agroecosystems
expand article infoLucija Šerić Jelaska, Barbara Anđelić§, Mišel Jelić|, Tomislav Kos, Hugo Benitez#
‡ University of Zagreb, Zagreb, Croatia
§ University of Zagreb, Faculty of Science, Department of Biology, Zagreb, Croatia
| Varaždin City Museum, Šetalište Josipa Jurja Strossmayera 3, Varaždin, Croatia
¶ University of Zadar, Department for ecology, agronomy and aquaculture, Zadar, Croatia
# Universidad de Tarapacá, Facultad de Ciencias, Departamento de Biología, Arica, Chile
Open Access


Neonicotinoids have been the most commonly used insecticides worldwide in the last two decades, whereas application of Copper (Cu) has a much longer history, especially in Mediterranean agriculture, (e.g. vineyards). Lately, large numbers of studies have shown negative effects of neonicotinoids on non-target species (especially on bees but also on other insects, and indirectly on insectivorous species like birds) being thus recently banned by EU commission. On the other hand, intensification of production in favour of ecological pest management increases the use of copper in pest control schemes and its absorption in agroecosystems. In this study we aimed to quantify the relationships between pesticide concentrations, including those with copper compounds, in soil, carabids and its prey at four sampling sites, in vineyards and olive groves under integrated (IPM) and ecological pest managements (EPM), and in pristine habitats in Zadar County, in Mediterranean region of Croatia. Soil, carabids, earthworms, snails and woodlice were sampled using pitfall traps and by hand. LC-MS/MS and ICP-MS technique were utilised to quantify pesticides and Cu concentrations in the soil and in the animals representing different trophic guilds. In addition we analysed changes in body shape symmetry of Poecilus koyi, one of the most dominant predatory carabid species in the study sites, in order to evaluate the potential environmental stress between maned and pristine habitats that can affect carabids during their development. Geometric morphometrics (GMM) has been used to analyse pronotum and abdomen shape variations, and left/right asymmetries in up to100 individuals of P. koyi per study site. Some preliminary results showed that body burden with pesticides differ between prey and predator organisms, and body concentrations of certain pesticides in carabid beetles did not correlate with those in the soil. Distribution of pesticide along different trophic guilds and changes in body shape and fluctuating asymmetry in P. koyi populations will be presented and discussed in details.


fluctuating asymmetry, olive orchards, predators, pest management type, pesticides, vineyards

Presenting author

Barbara Anđelić

Presented at

19thECM oral communication


This work has been supported by Croatian Science Foundation HRZZ under the project UIP-2017-05-1046, and the Department of Biology at the Faculty of Science, University of Zagreb.

Funding program

Installation Grant HRZZ UIP 05-2017-1046

Grant title

Neonicotinoids and Copper in the Mediterranean Agriculture – their effects on non-target invertebrates through trophic interactions

Hosting institution

University of Zagreb, Faculty of Science, Horvatovac 102A, Zagreb,Croatia

Conflicts of interest

Authors declare no conflicts of intrests.

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