ARPHA Conference Abstracts :
Conference Abstract
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Corresponding author: Laura García Abad (laura.garciaa@umh.es)
Received: 20 Feb 2021 | Published: 04 Mar 2021
© 2021 Laura García Abad, Alžběta Vondrášková, Antonia Dolores Asencio Martínez, Jan Kaštovský, Esther Berrendero Gómez
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
García Abad L, Vondrášková A, Asencio Martínez AD, Kaštovský J, Berrendero Gómez E (2021) Using single cell/filament PCR method for study non-cultured cyanobacteria. ARPHA Conference Abstracts 4: e64698. https://doi.org/10.3897/aca.4.e64698
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Cyanobacteria are photosynthetic prokaryotic organisms, and they are believed to be the Earth’s oldest life form. They were responsible for forming the oxygen on the Earth and from then they have been able to adapt to a multitude of environmental conditions, which allowing to be present in a wide range of habitats. When it comes to perform ecological and/or taxonomic studies one of problems which we must confront is the fact of some cyanobacteria are present in the nature in a few amounts, going unnoticed. Moreover, some of them show large mucilaginous sheaths, which making difficulted its isolation and growth later in culture. All these aspects have hindered the molecular assessment of a large portion of traditional cyanobacterial taxa, as Rivularia and Stigonema, especially necessary evaluation in the taxonomic studies. Rivularia genus grows on submerged stones, rocks, and damp soils near riverbanks, often on calcareous substrates and in clear, unpolluted, and streaming or stagnant waters, but also there are several species known from marine littoral. However, Stigonema genus grows aerophytically or subaerophytically on bark of trees or wet rocks, from lowland to the alpine zone of high mountains, but also several species are known from pools, swamps and moors. In this study, several Rivularia colonies collected in the Croatian Adriatic coast and several Stigonema species from Spanish alpine terrestrial environments are being analysed. The used methodology consists of a number of stages. In the first step, the samples are washed in EDTA disodium salt solution for dissolving the associated of calcium carbonate deposits (STEP 1). For the isolation of single cells and filaments, we are using a modified single filament isolation technique for planktonic cyanobacteria applying a glass capillary under sterile conditions (
16S rRNA, cyanobacteria, single cell PCR, taxonomy, Rivularia, Stigonema
Laura García Abad
1st DNAQUA International Conference (March 9-11, 2021)