ARPHA Conference Abstracts : Conference Abstract
Conference Abstract
Ciliates as bioindicators of environmental pressure in a karstic river 
expand article infoAntonija Kulaš, Vesna Gulin, Renata Matoničkin Kepčija, Petar Žutinić, Mirela Sertić Perić, Sandi Orlić§,|, Katarina Kajan§,|, Thorsten Stoeck, Guillaume Lentendu¶,#, Ivan Čanjevac¤, Ivan Martinić¤, Marija Gligora Udovič
‡ University of Zagreb, Faculty of Science, Department of Biology, Rooseveltov trg 6, HR-10000 Zagreb, Croatia
§ Ruđer Bošković Institute, Bijenička cesta 54, HR-10000 Zagreb, Croatia
| Center of Excellence for Science and Technology Integrating Mediterranean Region (STIM), HR-10000 Zagreb, Croatia
¶ University of Kaiserslautern, Ecology group, Erwin-Schrödinger-Strasse, D-67663 Kaiserslautern, Germany
# University of Neuchâtel, Institute of Biology, rue Emile Argand 11, CH-2000 Neuchâtel, Switzerland
¤ University of Zagreb, Faculty of Science, Department of Geography, Marulićev trg 19/II, HR-10000 Zagreb, Croatia
Open Access


Ciliates (protozoa) are a very large and diverse group of microeukaryotes that occupy a central position in the trophic web of freshwater ecosystems. Ciliates exhibit high ecological sensitivity and have tremendous bioindicator potential, but they are largely ignored in routine biomonitoring, mainly due to limitations in morphological diagnosis, which is both time-consuming and costly. In this study, we combined molecular (Illumina sequencing of the hypervariable V9 region of the SSU rRNA gene) and morphological approaches to get a detailed insight into the periphytic ciliate community structure and function within a karst, tufa-precipitating hydrosystem (National Park Krka, Croatia). Periphyton (biofilm) was sampled from light- and dark-exposed lithified tufa/stones at four representative locations within the Krka River, including upstream, midstream, and downstream river sections. We identified hydrological parameters and saprobiological classification of the sampling locations as the main structuring factors for ciliate communities. The molecular approach showed a clear separation of sampling locations in beta diversity analyses, as confirmed by PERMANOVA test. In contrast, alpha diversity was mostly affected by light exposition of the lithified tufa/stones, as confirmed by morphological approach. Environmental conditions consistently affected ciliate community composition at all locations, resulting in clear separation of periphyton samples. Our results indicate that ciliates should be considered as important bioindicators in monitoring tufa-forming rivers and streams, and the molecular approach (V9 region) proved to be applicable because as it efficiently captured the diversity of ciliates in periphyton. Validation of such an approach would lead to significant progress in extending monitoring to a broader range of indicator organisms than those now included in standard monitoring.


ciliates, karstic river, biofilm, eDNA metabarcoding, microscopy approach.

Presenting author

Antonija Kulaš

Presented at

1st DNAQUA International Conference (March 9-11, 2021)