ARPHA Conference Abstracts :
Conference Abstract
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Corresponding author: Manan Shah (manan.b.shah@gmail.com)
Received: 22 Feb 2021 | Published: 04 Mar 2021
© 2021 Manan Shah, Till Bornemann, Julia Nuy, Alexander Probst, Daniela Beisser, Jens Boenigk
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Shah M, Bornemann TLV, Nuy J, Probst A, Beisser D, Boenigk J (2021) Identifying genome level variation in generalist and specialist bacteria and their relationship to abiotic conditions in European freshwater ecosystems. ARPHA Conference Abstracts 4: e64807. https://doi.org/10.3897/aca.4.e64807
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Microbial biodiversity is one of the increasingly studied fields, yet we are unable to accurately pinpoint the intricacies of how the biotic landscape changes with its abiotic counterpart. Every organism reacts differently to physical changes in its surrounding(
We hypothesize that both generalists and specialists have developed advanced strategies that allow them thrive under diverse conditions or in very specific niches, and that these changes should be traceable on the genomic level. We expect to see variations in factors like genome sizes, number of genes, GC content, metabolic pathways that yield growth and replication advantages in nutrient deficient niches and defense mechanisms. We also expect to identify genomic streamlining in organisms that survive in nutrient deficient environments.
We are currently studying the genetic basis of why these generalists are so universally present though in smaller numbers, and what allows the specialists to efficiently exploit the abiotic factors of their specific environment in order to flourish. To pursue this, we have sampled and sequenced metagenomes from 47 lakes spread across Europe with strongly diverging environmental conditions such as pH, temperature, organic and nutrient content, elevation, and conductivity.
We’ve identified reads from more than 9000 taxonomically unique organisms, of which 700 were identified as generalists and 1200 as specialists, based on niche width permutations. In total we have assembled 313 high quality Metagenome Assembled Genomes (MAGs). Which now allow us to get a more detailed insight into the genetic basis and specific adaptations of these organisms.
Metagenomics, MAGs, Biodiversity, Generalists, Specialists
Manan Shah
1st DNAQUA International Conference (March 9-11, 2021)
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