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ARPHA Conference Abstracts :
Conference Abstract
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Jasmina Kamberović‡, Marija Gligora Udovič§, Maria Kahlert|, Kálmán Tapolczai¶, Zorana Lukić‡, Adisa Ahmić‡, Anita Dedić#, Antonija Kulaš§, Katarina Kajan¤,«, Jasna Hanjalić»,˄, Lejla Lasić˄,», Belma Kalamujic Stroil»,˄|
Corresponding author: Jasmina Kamberović (jasmina.kamberovic13@gmail.com)
Received: 24 Feb 2021 | Published: 04 Mar 2021
© 2021 Jasmina Kamberović, Marija Gligora Udovič, Maria Kahlert, Kálmán Tapolczai, Zorana Lukić, Adisa Ahmić, Anita Dedić, Antonija Kulaš, Katarina Kajan, Jasna Hanjalić, Lejla Lasić, Belma Kalamujic Stroil
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Kamberović J, Gligora Udovič M, Kahlert M, Tapolczai K, Lukić Z, Ahmić A, Dedić A, Kulaš A, Kajan K, Hanjalić J, Lasić L, Kalamujic Stroil B (2021) Composition of diatom communities on travertine barriers of the Una River (Bosnia and Herzegovina) obtained by DNA metabarcoding and morphological analysis. ARPHA Conference Abstracts 4: e64954. https://doi.org/10.3897/aca.4.e64954
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Abstract
The process of travertine formation and carbonate deposition in the rivers is unique, delicate, and depends on the activity of algae and mosses. Although diatoms have been used extensively in hydrobiological studies, the comparative analysis data on diatom communities of the travertine barriers in karstic rivers are still scarce.
The study aimed to detect the diatom composition on travertine barriers in the Una River, the large karstic river in Bosnia and Herzegovina. An integrated classical morphological identification approach with metabarcoding was applied on eight samples across the river length profile. Morphological analyses were performed using both light and scanning electron microscopes. Subsequent DNA metabarcoding of the chloroplastic gene 312bp rbcL was done. The DADA2 pipeline was used for the bioinformatic treatment of the demultiplexed MiSeq reads to infer Amplicon Sequence Variants (ASVs). ASVs were taxonomically assigned using the Diat.barcode v7 reference database.
A total of 126 species were identified using the morphological approach, while 133 ASVs were taxonomically assigned to 58 unique taxa with the molecular approach. Diatom community structures in terms of molecular and morphological approaches were congruent with 49 shared species. Species from genera Gomphonema, Navicula and Encyonema were less assigned in molecular analysis.
The most abundant taxa in the Una River are alkaliphilous, belonging to the genera Gomphonema, Nitzshia and Navicula. Although specific for their extremely good chemical status, the travertine barriers of the Una River are largely inhabited with meso-eutraphentic taxa.
Keywords
diatoms, travertine, biodiversity, morphological approach, metabarcoding, rbcL, Una River
Presenting author
Jasmina Kamberović
Presented at
1st DNAQUA International Conference (March 9-11, 2021)
Funding program
The conference announcement is the result of the STMS under the EU COST Action CA15219 DNAqua-Net: Preparation of the pilot project proposal on metabarcoding and morphological identification to assess diatom diversity in poorly explored Dinaric rivers hosted at SLU University in Sweden. The realization of the project activities is funded by Ministry of Civil Affairs of Bosnia and Herzegovina (project Pilot project of identification of benthic diatoms of the Una River using DNA metabarcoding), and by Federal Ministry of Education and Science of Bosnia and Herzegovina (project Application of diatoms indices in the assessment of the ecological status of the Una River).