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ARPHA Conference Abstracts :
Conference Abstract
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Micaela Hellstrom‡, Kat Bruce§, Rein Brys|, Bernd Hänfling¶, David Halfmaerten#, Cuong Tang¤, Jessica Sjöstedt«, Martin Andersson», Marcus Öhman», Johan Spens˄|
Corresponding author: Micaela Hellstrom (info@mixresearch.se)
Received: 25 Feb 2021 | Published: 04 Mar 2021
© 2021 Micaela Hellstrom, Kat Bruce, Rein Brys, Bernd Hänfling, David Halfmaerten, Cuong Tang, Jessica Sjöstedt, Martin Andersson, Marcus Öhman, Johan Spens
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Hellstrom M, Bruce K, Brys R, Hänfling B, Halfmaerten D, Tang C, Sjöstedt J, Andersson M, Öhman M, Spens J (2021) LifeDNAquatic: A priori settings and refined pipelines of eDNA metabarcoding to generate "big data" for solid fish, mussel and amphibian SDMs. ARPHA Conference Abstracts 4: e65052. https://doi.org/10.3897/aca.4.e65052
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Abstract
Sound environmental management decisions - in accordance with the EU WFD for aquatic ecosystems – mainly depend on reliable species presence- and distribution- data. Here we present a workflow from sampling strategies to results and decision making using eDNA metabarcoding analyses for fish, amphibians, and mussels from habitat to landscape scales with focus on sampling strategies for "big data" in marine and freshwater ecosystems in Sweden. The project LifeDNAquatic highlights a solid eDNA pipeline and comparison of methods, which cover field planning and the entire pipeline generating data for Species Distribution Models (SDMs). Intense sampling over a large river catchment highlights previoulsy unanswered questionsand and provides insights to a priori settings for sampling strategies to retrieve "big data". The results provide novel insights to DNA distribution in the environment, seasonal and spatial changes in eDNA composition, and validation of data.
Keywords
aquatic eDNA, metabarcoding, method validation, storage,"big data", landscape scale, Species Distribution Models, a priori, predictions, novel spatial and seasonal eDNA distribution, mussels, amphibians, marine, wetland, lentic, lotic
Presenting author
Dr Micaela Hellström, MIX Research
Presented at
1st DNAQUA International Conference (March 9-11, 2021)
Funding program
Swedish Environmental Agency , Swedish Agency for Marine and Water Management call: "eDNA anayses as a method for national environmental monitoring programs" 2019-2021
Grant title
LifeDNAquatics
Hosting institution
AquaBiota Water Research AB
Ethics and security
NA
Author contributions
MH, KB, JN, BH, RB, DH concept, field design and method comparison design, analysis, writing,
MH, KB, JS, JeS, MA - fieldwork
CT, JeS - eDNA extractions
CT - Metabarcoding and NGS, NGS data analysis
JoS - background data, species distribution analysis
MÖ - discussions, administrative coordinator