ARPHA Conference Abstracts :
Conference Abstract
|
Corresponding author: Valentin Vasselon (valentin.vasselon@scimabio-interface.fr)
Received: 26 Feb 2021 | Published: 04 Mar 2021
This is an open access article distributed under the terms of the CC0 Public Domain Dedication.
Citation:
Vasselon V, Ács É, Almeida S, Andree K, Apothéloz-Perret-Gentil L, Bailet B, Baricevic A, Beentjes K, Bettig J, Bouchez A, Capelli C, Chardon C, Duleba M, Elersek T, Genthon C, Hurtz M, Jacas L, Kahlert M, Kelly M, Lewis M, Macher JN, Mauri F, Moletta-Denat M, Mortágua A, Pawlowski J, Pérez Burillo J, Pfannkuchen M, Pilgrim E, Pissaridou P, Porter J, Rimet F, Stanic K, Tapolczai K, Theroux S, Trobajo R, van der Hoorn B, Vasquez Hadjilyra MI, Walsh K, Wanless D, Warren J, Zimmermann J, Zupančič M (2021) The Fellowship of the Ring Test: DNAqua-Net WG2 initiative to compare diatom metabarcoding protocols used in routine freshwater biomonitoring for standardisation. ARPHA Conference Abstracts 4: e65142. https://doi.org/10.3897/aca.4.e65142
|
During the past decade genetic approaches have been developed to monitor biodiversity in aquatic ecosystems. These enable access to taxonomic and genetic information from biological communities using DNA from environmental samples (e.g. water, biofilm, soil) and methods based on high-throughput sequencing technologies, such as DNA metabarcoding. Within the context of the Water Framework Directive (WFD), such approaches could be applied to assess Biological Quality Elements (BQE). These are used as indicators of the ecological status of aquatic ecosystems as part of national monitoring programs of the european network of 110,000 surface water monitoring sites with 79.5% rivers and 11% lake sites (
Good progress has been made with developing DNA metabarcoding approaches for benthic diatom assemblages. Technological innovation and protocol optimization have allowed robust taxonomic (species) and genetic (OTU, ESV) information to be obtained from which diatom quality indices can be calculated to infer ecological status to rivers and lakes. Diatom DNA metabarcoding has been successfully applied for biomonitoring at the scale of national river monitoring networks in several countries around the world and can now be considered technically ready for routine application (e.g.
Following previous initiatives which resulted in a CEN technical report for biofilm sampling and preservation (
DNA Metabarcoding, Diatom, Intercalibration, Ring test
Valentin Vasselon
1st DNAQUA International Conference (March 9-11, 2021)