ARPHA Conference Abstracts :
Conference Abstract
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Corresponding author: Sean Markus (s.markus.19@abdn.ac.uk), Iveta Matejusova (iveta.matejusova@gov.scot), Alex Douglas (a.douglas@abdn.ac.uk), William Sanderson (w.g.sanderson@hw.ac.uk)
Received: 02 Mar 2021 | Published: 04 Mar 2021
© 2021 Sean Markus, Iveta Matejusova, Alex Douglas, William Sanderson
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Markus S, Matejusova I, Douglas A, Sanderson W (2021) Development of eDNA tools for the detection of marine invasive non-native species to support European flat oyster (Ostrea edulis) restoration projects. ARPHA Conference Abstracts 4: e65383. https://doi.org/10.3897/aca.4.e65383
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The European flat oyster (Ostrea edulis) is an important keystone species in Scottish coastal waters. However due to anthropogenic pressures, significant reductions to oyster beds have been observed across Europe. In Scotland, several projects are currently aiming to restore European flat oyster habitats through the translocation of juvenile oysters from various sources including hatcheries and aquaculture. However, translocation of shellfish is not risk free and can increase the risk of accidental translocation of invasive non-native species (INNS). If INNS become established outside of their native range they can cause irreversible harm to native organisms and habitats. This study aims to develop molecular tools to detect environmental DNA of INNS which can be potentially associated with the translocation of live shellfish stocks. We have developed a species-specific real-time PCR assay for detection of Pacific oyster (Crassostrea gigas) and tested its sensitivity in a large-scale replicated mesocosm based experiment with varying densities of C.gigas. A secondary objective of the experiment was to assess the detection of another invasive species, the carpet sea squirt Didemnum vexillum which was cohabited with C. gigas. We aim to quantify the detection probability of increasing densities of C. gigas from repeat water samples and qPCR replicates. This project also aims to investigate the feasibility of using portable, real-time sequencing technologies such as the Oxford Nanopore MinION to develop robust tools to support native oyster restoration programmes.
Environmental DNA (eDNA)
Invasive non-native species (INNS)
qPCR
Oxford Nanopore MinION
Sean Markus
1st DNAQUA International Conference (March 9-11, 2021)
Alex Douglas1 , William Sanderson2 and Iveta Matejusova3
1School of Biological Sciences, University of Aberdeen, Aberdeen, AB24 2TZ, UK
2 Centre for Marine Biodiversity & Biotechnology, ILES, Heriot-Watt University, Edinburgh, EH14 4AS, UK
3Marine Scotland Science, Marine Laboratory, Victoria Road, Aberdeen, AB11 9DB, UK
SUPER DTP
University of Aberdeen
Alex Douglas1 , William Sanderson2 and Iveta Matejusova3
1School of Biological Sciences, University of Aberdeen, Aberdeen, AB24 2TZ, UK
2 Centre for Marine Biodiversity & Biotechnology, ILES, Heriot-Watt University, Edinburgh, EH14 4AS, UK
3Marine Scotland Science, Marine Laboratory, Victoria Road, Aberdeen, AB11 9DB, UK