ARPHA Conference Abstracts :
Conference Abstract
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Corresponding author: Juan Antonio Villaescusa (juanantonio.villaescusa@ltlevante.com)
Received: 02 Mar 2021 | Published: 04 Mar 2021
© 2021 Juan Antonio Villaescusa, Mª José Villena, Raquel González, Antonio Picazo, Verónica Rojo, Marko Nieminem, Silke Classen, Ana Pujante, Antonio Camacho
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Villaescusa JA, Villena MJ, González R, Picazo A, Rojo V, Nieminem M, Classen S, Pujante A, Camacho A (2021) Macroinvertebrate community assessment and biomonitoring of European water bodies. Is a multimarker approach necessary? ARPHA Conference Abstracts 4: e65390. https://doi.org/10.3897/aca.4.e65390
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The present work, included in the European Project BIOWAT, aims to develop and validate the use of genomic tools or metabarcoding for its utilization as a routine method for river biomonitoring in different European Biogeographical regions. The project included sampling points in three biogeographic regions, Mediterranean (Spain), Continental (Germany) and Boreal (Finland). The current development of the study was designed using mock communities obtained from the three mentioned areas and different aspects were tested: DNA extraction methods, selection of informative region (16S vs COI), design and performance of primers, bioinformatic pipeline, etc…
Although the use of COI has become very popular, and its barcode database is more complete, the use of mitochondrial 16S as taxonomic marker can provide similar or even better results when accompanied by a rich local barcode database
Additionally, for the studied Mediterranean rivers, a complementary analysis using COI as marker was made, using the universal primers developed by
Our results show that both markers have the potential to produce a good identification of benthic macroinvertebrates, showing an acceptable correlation between morphology and metabarcoding approaches. However, none of them is able to amplify all of the present groups, so the parallel use of both markers (mitochondrial 16S and COI) in a multimarker approach could solve some of the problems, giving a more complete profile of the macroinvertebrate community. This approach has already been proposed and can lead the future of macroinvertebrate community assessment
metabarcoding, benthic macroinvertebrates, mitochondrial 16S, COI, biomonitoring
Juan Antonio Villaescusa
1st DNAQUA International Conference (March 9-11, 2021)