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Conference Abstract
Amplicon Sequencing in the Era of Highly-Accurate Long Reads
expand article info Benjamin J Callahan ‡
‡ North Carolina State University, Raleigh, United States of America
Corresponding author: Benjamin J Callahan (benjamin.j.callahan@gmail.com)
Received: 02 Mar 2021 | Published: 04 Mar 2021
© 2021 Benjamin Callahan
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation: Callahan BJ (2021) Amplicon Sequencing in the Era of Highly-Accurate Long Reads. ARPHA Conference Abstracts 4: e65405. https://doi.org/10.3897/aca.4.e65405
 Open Access

Abstract

An important advance in DNA sequencing has been the development of long-read sequencing technologies that produce sequencing reads of tens to hundreds of kilobases in length. However, these technologies typically have high (~8%) per-base error rates. Recently, an effectively new technology I call highly-accurate long-read sequencing has been developed, that allows for the generation of multi-kilobase reads with extremely high per-base accuracies (>99.9%). I will present and evaluate two such technologies, PacBio HiFi and LoopSeq SLR sequencing, and discuss potential metabarcoding applications of highly-accurate long-read amplicon sequencing in general.

Keywords

amplicon sequencing, long reads, DNA sequencing, metabarcoding, NGS

Presenting author

Benjamin Callahan, North Carolina State University, Department of Population Health and Pathobiology, USA

Presented at

1st DNAQUA International Conference (March 9-11, 2021)