ARPHA Conference Abstracts : Conference Abstract
Conference Abstract
Amplicon Sequencing in the Era of Highly-Accurate Long Reads
expand article info Benjamin J Callahan
‡ North Carolina State University, Raleigh, United States of America
Open Access


An important advance in DNA sequencing has been the development of long-read sequencing technologies that produce sequencing reads of tens to hundreds of kilobases in length. However, these technologies typically have high (~8%) per-base error rates. Recently, an effectively new technology I call highly-accurate long-read sequencing has been developed, that allows for the generation of multi-kilobase reads with extremely high per-base accuracies (>99.9%). I will present and evaluate two such technologies, PacBio HiFi and LoopSeq SLR sequencing, and discuss potential metabarcoding applications of highly-accurate long-read amplicon sequencing in general.


amplicon sequencing, long reads, DNA sequencing, metabarcoding, NGS

Presenting author

Benjamin Callahan, North Carolina State University, Department of Population Health and Pathobiology, USA

Presented at

1st DNAQUA International Conference (March 9-11, 2021)