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Conference Abstract
Development of species-specific Cichla species eDNA primers for alien invasive species (AIS) monitoring in Malaysia
expand article infoO. Nurul Fizatul Nabilah‡, A. R. Ramizah‡, A. B. Adibah‡, S. Syazwan‡, A.G. Intan Faraha§, A. Amirrudin|, M. N. Siti Azizah
‡ Department of Biology, Faculty of Science and Mathematics, Universiti Pendidikan Sultan Idris (UPSI), 35900, Tanjong Malim, Perak., Tanjung Malim, Malaysia
§ Department of Science and Biotechnology, Faculty of Engineering and Life Sciences, 45600, Bestari Jaya, Selangor., Selangor, Malaysia
| School of Marine and Environmental Sciences, Universiti Malaysia Terengganu, 21030, Kuala Terengganu, Terengganu, Malaysia., Terengganu, Malaysia
¶ Institut Bioteknologi Marin, Universiti Malaysia Terengganu, 21030, Kuala Terengganu, Terengganu, Malaysia., Terengganu, Malaysia
Corresponding author: O. Nurul Fizatul Nabilah (nurulfizatul94@gmail.com), A. R. Ramizah (ramizahrahman96@gmail.com), A. B. Adibah (adibah@fsmt.upsi.edu.my), S. Syazwan (syazwan@fsmt.upsi.edu.my), A.G. Intan Faraha (intanfaraha@unisel.edu.my), A. Amirrudin (amirudin@umt.edu.my), M. N. Siti Azizah (azizahpfl@yahoo.com)
Received: 03 Mar 2021 | Published: 04 Mar 2021
This is an open access article distributed under the terms of the CC0 Public Domain Dedication.
Citation: Nurul Fizatul Nabilah O, Ramizah AR, Adibah AB, Syazwan S, Intan Faraha A.G, Amirrudin A, Siti Azizah MN (2021) Development of species-specific Cichla species eDNA primers for alien invasive species (AIS) monitoring in Malaysia. ARPHA Conference Abstracts 4: e65449. https://doi.org/10.3897/aca.4.e65449
 Open Access

Abstract

Peacock bass or the cichlids are known locally as top predator fishes which are invasive in Malaysia freshwater system. Detection probabilities for these fishes are typically low, especially using conventional capture-survey method due to the fish’s behaviour of hiding beneath the water’s surface. Hence, the environmental DNA (eDNA) monitoring is a relatively new approach that can be used to assess the distribution of these invasive fishes. Here, we report the strategy to develop small fragment (280- 400 bp) specific-specific primers for three selected invasive Cichla species namely, C. ocellaris, C. monoculus, and C. kelberi based on mitochondrial DNA (mtDNA) sequences. Current research showed that the developed species-specific primers from cytochrome oxidase I (COI) gene has high resolution at species level. Species-specific amplification tests also proved the specificity of the developed primers, securing the high- level species identification potential which may help in controlling the spread of alien invasive fish species.

Keywords

Species- specific primer, COI primer, eDNA marker, invasive species.

Presenting author

Nurul Fizatul Nabilah Osman

Presented at

1st DNAQUA International Conference (March 9-11, 2021)