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ARPHA Conference Abstracts :
Conference Abstract
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Natacha Nikolic‡,§, Emmanuel Corse|, Nicolas Juillet¶|
Corresponding author: Natacha Nikolic (natacha.nikolic@inrae.fr)
Received: 03 Mar 2021 | Published: 04 Mar 2021
© 2021 Natacha Nikolic, Emmanuel Corse, Nicolas Juillet
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Nikolic N, Corse E, Juillet N (2021) Ichtyological diversity in a tropical nature reserve (Etang de Saint-Paul, Réunion Island) from environmental DNA (eDNA) metabarcoding. ARPHA Conference Abstracts 4: e65462. https://doi.org/10.3897/aca.4.e65462
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Abstract
The aim of this study was to test whether the ichthyological diversity of one natural reserve in Reunion Island (Réserve de l'Etang de Saint-Paul) could be established with a molecular tool, environmental DNA (eDNA). We hence filtrated the water (2L) at 10 different areas around the reserve. For each sampling area, 12 PCR replicas were performed and the identification of fish species was carried out by metabarcoding through a primer over a mitochondrial region (12S). This study showed the importance of reference sequencing databases as well as improvements through phylogenetic analyses. This first fish study by eDNA in La Réunion also revealed the coherence of the distribution of species and their habitat.
See the Poster in Suppl. material
Collection : 1st DNAQUA International Conference - poster session.
Keywords
Fish, tropical, eDNA, metabarcoding, diversity,protected area
Presenting author
Natacha Nikolic - Researcher at INRAE; PhD Ecology, Biodiversity, and Evolution.
Emmanuel Corse - MCF at University of Mayotte; PhD Molecular Ecology.
Nicolas Juillet - Past scientific manager at RNNESP and currently project manager at Ecologistes de l'Euziere; PhD in Ecology.
Presented at
1st DNAQUA International Conference (March 9-11, 2021)
Funding program
Funding: Réserve naturelle nationale de l'étang de Saint-Paul (RNNESP)
Grant title
National by the RNNESP
Author contributions
NN and NJ design the project. NN and NJ made the sampling. Spygen made the DNA extraction, DNA amplification and obitools biofiltration. NN and EC made the genetic analysis. NN, EC, and NJ interpretated the results. NN, EC, and NJ wrote the abstract and poster.
Conflicts of interest
None conflicts of interest.