Environmental DNA methods for the analysis of macroorganismal populations

Hideyuki Doi

doi:10.3897/aca.4.e65549

ARPHA Conference Abstracts : Conference Abstract

Conference Abstract

Environmental DNA methods for the analysis of macroorganismal populations

Hideyuki Doi ^‡

‡ University of Hyogo, Kobe, Japan

Corresponding author: Hideyuki Doi (hideyuki.doi@icloud.com)

Received: 05 Mar 2021 | Published: 08 Mar 2021

This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Citation: Doi H (2021) Environmental DNA methods for the analysis of macroorganismal populations. ARPHA Conference Abstracts 4: e65549. https://doi.org/10.3897/aca.4.e65549

Abstract

Environmental DNA (eDNA) methods have been widely used to investigate the distribution and abundance/biomass of macroorganisms. eDNA methods analyze DNA collected directly from the environment, such as from water, soil, and air. The techniques have been applied to many taxa inhabiting various aquatic and terrestrial ecosystems. The recent development of eDNA methods has revolutionized the way we assess macroorganisms in natural environments.

In this talk, I will present current developments of eDNA methodology, especially with regard to population analysis using various DNA measurement methods. For example, 1) eDNA was used to assess fish species distributions and abundance/biomass (Takahara et al. 2012, Doi et al. 2017a), 2) quantitative PCR of sedimentary DNA was applied to sediment core samples to detect the DNA of three dominant fish species spanning the last 300 years (Kuwae et al. 2020), and 3) new methods for sampling eDNA from water (Doi et al. 2017b) and on-site measurement (Doi et al. 2020) will be presented. I end by addressing the need for standardized protocols for eDNA monitoring to enable broader uptake of eDNA technology (Minamoto et al. 2021).

Presenting author

Hideyuki Doi

Presented at

1st DNAQUA International Conference (March 9-11, 2021)

Acknowledgements

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References

Doi H, Inui R, Akamatsu Y, Kanno K, Yamanaka H, Takahara T, Minamoto T (2017a)

Environmental DNA analysis for estimating the abundance and biomass of stream fish

Freshwater Biology

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Doi H, Akamatsu Y, Watanabe Y, Goto M, Inui R, Katano I, Nagano M, Takahara T, Minamoto T (2017b)

Water sampling for environmental DNA surveys by using an unmanned aerial vehicle

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Doi H, Watanabe T, Nishizawa N, Saito T, Nagata H, Kameda Y, Maki N, Ikeda K, Fukuzawa T (2020)

On-site eDNA detection of species using ultra-rapid mobile PCR

BioRxiv

https://doi.org/10.1101/2020.09.28.314625

Kuwae M, Tamai H, Doi H, Sakata M, Minamoto T, Suzuki Y (2020)

Sedimentary DNA tracks decadal-centennial changes in fish abundance

Communications Biology

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. https://doi.org/10.1038/s42003-020-01282-9

Minamoto T, Miya M, Sado T, Seino S, Doi H, Kondoh M, Nakamura K, Takahara T, Yamamoto S, Yamanaka H, Araki H, Iwasaki W, Kasai A, Masuda R, Uchii K (2021)

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Takahara T, Minamoto T, Yamanaka H, Doi H, Kawabata Z (2012)

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Supplementary material

Endnotes