ARPHA Conference Abstracts : Conference Abstract
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Conference Abstract
Catmint (Nepeta nuda L.) phylogenetics defined by nuclear and chloroplast DNA barcodes
expand article infoGeorgi Bonchev‡, Miroslava Zhiponova§, Anita Tosheva|, Valya Vassileva‡
‡ Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, Sofia, Bulgaria
§ Department of Plant Physiology, Faculty of Biology, Sofia University “St. Kliment Ohridski”, Sofia, Bulgaria
| Department of Botany, Faculty of Biology, Sofia University “St. Kliment Ohridski”, Sofia, Bulgaria
Corresponding author: Georgi Bonchev (georgi.bonchev71@gmail.com)
Received: 03 Jun 2022 | Published: 17 Jun 2022
© 2022 Georgi Bonchev, Miroslava Zhiponova, Anita Tosheva, Valya Vassileva
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation: Bonchev G, Zhiponova M, Tosheva A, Vassileva V (2022) Catmint (Nepeta nuda L.) phylogenetics defined by nuclear and chloroplast DNA barcodes . ARPHA Conference Abstracts 5: e87343. https://doi.org/10.3897/aca.5.e87343
 Open Access

Abstract

Nepeta nuda L. (Lamiaceae) is a medicinal plant with a wide distribution in Europa and Asia. In Bulgaria, N. nuda is also known as “naked (or hairless) catmint”, which likely refers to the naked or sparse short hairy stem and leaves. This study aims to generate DNA barcodes for precise genetic discrimination and determination of phylogenetic position of the plant among other Nepeta species. To achieve this goal, we applied the DNA barcoding technique based on conserved nuclear (internal transcribed spacer/ITS) and chloroplast (rbcL, matK, trnH) DNA regions. The generated DNA barcode sequences were submitted to the Barcode of Life Data System (BOLD) database (https://www.boldsystems.org; accession number BUL002-22). The obtained N. nuda DNA barcodes and corresponding sequences of other Nepeta species available in the BOLD and NCBI database were utilized for taxonomic classification. ITS-derived sequences for Nepeta species were the most enriched in the database, and DNA fragments matching 47 Nepeta species were selected for construction of phylogenetic tree. The results show that N. nuda is clustered in a subclade together with N. sheilae, N. deflersiana, N. isaurica, N. congesta, N. heliotropifolia, N. schiraziana and N. cataria. The information in BOLD was also retrieved for rbcL and matK, and corresponded to 15 and 10 different Nepeta species, respectively. For trnH, only NCBI sequences corresponding to 6 different Nepeta species were found. Тhe three chloroplast markers highlighted the close relation of N. nuda to N. italica, N. tuberosa, N. cataria, N. grandiflora and N. hemsleyana. In conclusion, we suggest a DNA barcode system for genetic discrimination of N. nuda, which could assist its accurate taxonomic characterization.

Keywords

DNA barcodes, genetic discrimination, Nepeta nuda, phylogenetic position

Presenting author

Georgi Bonchev

Presented at

International Conference on DNA Barcoding and Biodiversity 2022

Funding program

This work was financially supported by the Bulgarian National Science Fund (BNSF), Grant No. KP-06-N56/9/12.11.2021 and by the Ministry of Education and Science of Bulgaria, project BULCode No. Д01-271/02.10.2020, National Program “European Scientific Networks” 2020-2022

Hosting institution

Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences

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