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DNA BARCODE REFERENCES - The endless quest for completeness and curation of reference libraries
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Edited by Fedor Čiampor, Torbjørn Ekrem, Alex Weigand, Agnès Bouchez, Florian Leese

DNA barcode reference libraries are of immense importance for correct identification of organisms using DNA barcoding and metabarcoding. In this session we welcome contributions that focus on opportunities and challenges related to construction of reference databases and analysis of reference data: from filling the gaps and quality control of existing barcode libraries, to the analyses of species boundaries and use of integrative taxonomy in revision of classifications.

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Part of collection 1st DNAQUA International Conference 2021
Conference Abstract
Venturing into auditing of reference libraries: from the hackathon on marine invertebrates to sorting with BAGS
    Filipe Costa
10.3897/aca.4.e65504
 04-03-2021
 Unique: 1123 | Total: 1594  | Access Period:
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Conference Abstract
DNA barcoding reveals an unknown Chironomidae diversity from the freshwater biodiversity hot-spot: comparison between local and the European datasets
    Piotr Gadawski,     Matteo Montagna,     Bruno Rossaro, Wojciech Giłka,     Vladimir Pesic,     Michal Grabowski, Giulia Magoga
10.3897/aca.4.e65498
 04-03-2021
 Unique: 1402 | Total: 2037  | Access Period:
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Conference Abstract
DNA barcode library revealed unknown diversity of chironomid midges in Montenegro
    Piotr Gadawski,     Bruno Rossaro, Wojciech Giłka,     Tomasz Rewicz, Giulia Magoga,     Paul Hebert,     Matteo Montagna,     Michal Grabowski
10.3897/aca.4.e65497
 04-03-2021
 Unique: 1167 | Total: 1638  | Access Period:
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Conference Abstract
DNA Barcoding approach reveals 17 exploited fish species, including an unrecognised species of the yellow-lined snapper complex
ADIBAH ABU BAKAR, Siti Mohd Nor
10.3897/aca.4.e65494
 04-03-2021
 Unique: 1059 | Total: 1500  | Access Period:
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Conference Abstract
Mind the gap-analysis! – How complete are DNA barcode reference libraries for monitoring-relevant aquatic species in Europe?
Hannah Weigand
10.3897/aca.4.e65473
 04-03-2021
 Unique: 1176 | Total: 1628  | Access Period:
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Conference Abstract
Effects of anthropogenic pressures on diatom communities using morphological and molecular approaches
Marco Teixeira,     Salomé Almeida,     Maria Feio,     Andreia Mortágua, Manuela Sales
10.3897/aca.4.e64998
 04-03-2021
 Unique: 1107 | Total: 1632  | Access Period:
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Conference Abstract
DNA barcodes for UK freshwater arthropod species: coverage, quality and implications
Liz Davidson
10.3897/aca.4.e65255
 04-03-2021
 Unique: 1043 | Total: 1488  | Access Period:
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Conference Abstract
Establishing the first DNA barcode reference library for freshwater decapod species (Crustacea: Decapoda) in Republic of North Macedonia
Valentina Slavevska Stamenkovic, Jelena Hinic,     Michal Grabowski,     Tomasz Mamos, Leona Lovrenčić, Mišel Jelić, Goran Klobučar, Ivana Maguire
10.3897/aca.4.e65138
 04-03-2021
 Unique: 1090 | Total: 1578  | Access Period:
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Conference Abstract
First steps towards a barcoding database for the Iberian cladocerans
    Charo López-Blanco, Antonio García-Alix, Yi Wang, Laura S. Epp
10.3897/aca.4.e65122
 04-03-2021
 Unique: 1273 | Total: 1898  | Access Period:
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Conference Abstract
A reliable DNA barcode reference library for the identification of benthic invertebrates: essential for biomonitoring of the North Sea
    Magdalini Christodoulou, Berry van der Hoorn, Laure Van den Bulcke,     Sofie Derycke, Annelies De Backer, Ingrid Kröncke, Pedro Martinez Arbizu
10.3897/aca.4.e65063
 04-03-2021
 Unique: 1206 | Total: 1774  | Access Period:
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Conference Abstract
Community metabarcoding uncovers vast diversity and a lack of barcode references for aquatic invertebrates in Carpathian spring fens
    Magdalena Gajdošová,     Arne Beermann, Jindřiška Bojková, Vendula Polášková, Jana Schenková, Marie Zhai, Michal Horsák,     Florian Leese,     Adam Petrusek
10.3897/aca.4.e65057
 04-03-2021
 Unique: 1206 | Total: 1830  | Access Period:
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Conference Abstract
Filling gaps in the DNA barcode library - Aquatic insects (Ephemeroptera, Plecoptera, Trichoptera) from semi-mountainous and mountainous rivers of Ecoregion 6 (North Macedonia)
Biljana Rimcheska,     Yanka Vidinova
10.3897/aca.4.e65053
 04-03-2021
 Unique: 1003 | Total: 1449  | Access Period:
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Conference Abstract
Opportunities for collaboration between taxonomists and geneticists to database curation
    Mikel Aguirre, Joana Hevia-Orube, Laura Ocabo, Jesús Arrate, Eva López, Jose Manuel Leonardo, Alberto Aguirre, Begoña Gartzia de Bikuña
10.3897/aca.4.e65051
 04-03-2021
 Unique: 1107 | Total: 1605  | Access Period:
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Conference Abstract
Towards an eDNA assay for decapodiform cephalopods
    Gustavo Sanchez, Fernando Fernández-Álvarez
10.3897/aca.4.e65037
 04-03-2021
 Unique: 1114 | Total: 1619  | Access Period:
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Conference Abstract
Morphological features of larvae of Drusus osogovicus Kumanski, 1980 (Insecta, Trichoptera) from the Republic of North Macedonia, DNA barcoding of the species and notes on its ecology and distribution
Jelena Hinic, Valentina Slavevska Stamenkovic,     Halil Ibrahimi, Mladen Kučinić,     Julijana Arsovska, Milica Ristovska,     Florian Leese,     Arne Beermann
10.3897/aca.4.e65035
 04-03-2021
 Unique: 1218 | Total: 1837  | Access Period:
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Conference Abstract
DNA barcoding in recognition of Gammarusflock diversity and distribution in the ancient Lake Ohrid
    Anna Wysocka,     Michal Grabowski, Lidia Sworobowicz, Sasho Trajanovski,     Tomasz Mamos
10.3897/aca.4.e65011
 04-03-2021
 Unique: 1100 | Total: 1652  | Access Period:
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Conference Abstract
CO1 Metabarcoding of Marine Water Samples from the 2nd Turkish Antarctic Expedition (TAE2) Highlights the Deficiencies in the Reference Barcoding Databases for the Continent
    Rasit Bilgin, Kübra Karaman, Yağmur Tarhana, Ceylan Yücel, Elizabeth Hemond, Mehmet Yokeş, Evrim Kalkan,     Selahattin Karhan
10.3897/aca.4.e64987
 04-03-2021
 Unique: 1232 | Total: 1868  | Access Period:
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Conference Abstract
Stones in the road prevents effective implementation of eDNA-based freshwater quality monitoring in the Iberian Peninsula
    Álvaro Fueyo, Javier Granero-Castro, Beatriz Villazán Peñalosa, Yaisell Borrell Pichs
10.3897/aca.4.e64974
 04-03-2021
 Unique: 1194 | Total: 1803  | Access Period:
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Conference Abstract
Bacteria are everywhere, even in your COI data: Τhe art of getting to know the unknown unknowns and shine light on the dark matter!
    Haris Zafeiropoulos, Laura Gargan,     Christina Pavloudi, Evangelos Pafilis,     Jens Carlsson
10.3897/aca.4.e64966
 04-03-2021
 Unique: 1359 | Total: 2032  | Access Period:
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Conference Abstract
Gap analysis for DNA barcodes of aquatic macroinvertebrate species in the Southeast of Italy
Valeria Specchia,     Francesco Zangaro,     Eftychia Tzafesta,     Maurizio Pinna
10.3897/aca.4.e64956
 04-03-2021
 Unique: 1173 | Total: 1897  | Access Period:
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Conference Abstract
The value of DNA barcoding in a hotspot area: an example of Rhyacophila tristis (Trichoptera) in the Western Carpathians
    Jana Bozáňová,     Fedor Čiampor Jr,     Tomasz Mamos,     Michal Grabowski,     Zuzana Čiamporová-Zaťovičová
10.3897/aca.4.e64952
 04-03-2021
 Unique: 1188 | Total: 1838  | Access Period:
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Conference Abstract
Dots on Earth with huge genetic diversity: relict flatworm Crenobia alpinain the alpine lakes of Tatra Mountains
Kornélia Tuhrinová,     Jana Bozáňová,     Fedor Čiampor Jr,     Zuzana Čiamporová-Zaťovičová
10.3897/aca.4.e64946
 04-03-2021
 Unique: 1069 | Total: 1606  | Access Period:
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Conference Abstract
New kid on the block: Research of alpine-lake macroinvertebrates in the Tatra Mountains enhanced by DNA barcoding and metabarcoding
Ondrej Vargovčík,     Zuzana Čiamporová-Zaťovičová,     Fedor Čiampor Jr
10.3897/aca.4.e64945
 04-03-2021
 Unique: 1111 | Total: 1663  | Access Period:
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Conference Abstract
How many Non-Indigenous Species occurring in the Mediterranean Sea can actually be identified through environmental DNA? A gap analysis of NIS DNA barcodes in the reference libraries
    Maurizio Pinna,     Francesco Zangaro,     Eftychia Tzafesta, Valeria Specchia
10.3897/aca.4.e64943
 04-03-2021
 Unique: 1096 | Total: 1624  | Access Period:
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Conference Abstract
Diat.barcode: a DNA tool to decipher diatom communities for the evaluation environmental pressures
    Frederic Rimet, Teofana Chonova, Gilles Gassiole,     Maria Kahlert,     François Keck, Martyn Kelly, Maxim Kulikovskiy, David Mann,     Martin Pfannkuchen,     Ana Baričević,     Rosa Trobajo,     Valentin Vasselon, Jonas Zimmermann,     Carlos Wetzel,     Agnès Bouchez
10.3897/aca.4.e64940
 04-03-2021
 Unique: 1653 | Total: 2656  | Access Period:
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Conference Abstract
AquaBOL.SK - how far have we come with the Slovak reference barcode database since DNAqua-Net was launched?
    Zuzana Čiamporová-Zaťovičová, Patrik Macko, Michaela Šamulková, Ondrej Vargovčík,     Jana Bozáňová,     Fedor Čiampor Jr
10.3897/aca.4.e64939
 04-03-2021
 Unique: 1080 | Total: 1616  | Access Period:
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Conference Abstract
The small polychaete Platynereis dumerilii revealed as a large species complex with fourteen MOTUs in European marine habitats
    Marcos A. L. Teixeira, Arne Nygren,     Ascensão Ravara,     Pedro E. Vieira, José Carlos Hernández,     Filipe O. Costa
10.3897/aca.4.e64937
 04-03-2021
 Unique: 1332 | Total: 2010  | Access Period:
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Conference Abstract
From DNA sequences to operational reference databases: an opinionated approach using R
    François Keck,     Florian Altermatt
10.3897/aca.4.e64936
 04-03-2021
 Unique: 1191 | Total: 1783  | Access Period:
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Conference Abstract
GAPeDNA: Assessing and mapping global species gaps in genetic databases for metabarcoding studies
    Virginie Marques, Tristan Milhau, Camille Albouy, Tony Dejean, Stéphanie Manel, David Mouillot, Jean-Baptiste Juhel
10.3897/aca.4.e64884
 04-03-2021
 Unique: 1227 | Total: 1919  | Access Period:
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Conference Abstract
Conservation genetics of the vulnerable stone crayfish using DNA barcoding and microsatellites
Leona Lovrenčić, Lena Bonassin,     Frederic Grandjean, Chris Austin, Ivana Maguire
10.3897/aca.4.e64816
 04-03-2021
 Unique: 1202 | Total: 1845  | Access Period:
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Conference Abstract
DNA barcode reference libraries: Achievements of the DNAqua-Net Working Group 1
    Torbjørn Ekrem,     Fedor Čiampor Jr
10.3897/aca.4.e64811
 04-03-2021
 Unique: 1031 | Total: 1646  | Access Period:
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Conference Abstract
Challenges in building barcode reference libraries for marine invertebrates exemplified by the genus Prionospio (Annelida: Spionidae)
Martin Hektoen,     Torbjørn Ekrem,     Torkild Bakken
10.3897/aca.4.e64799
 04-03-2021
 Unique: 1190 | Total: 1868  | Access Period:
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Conference Abstract
The first step towards a DNA barcode reference database for mayflies (Ephemeroptera) of Slovakia
Patrik Macko, Tomáš Derka,     Fedor Čiampor Jr,     Zuzana Čiamporová-Zaťovičová
10.3897/aca.4.e64798
 04-03-2021
 Unique: 1079 | Total: 1722  | Access Period:
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Conference Abstract
Designing robust DNA barcode libraries for metabarcoding of freshwater plants by integrating herbarium collections and contemporary floristic inventories
    Armando Espinosa Prieto, Laurent Hardion, Jean-Nicolas Beisel
10.3897/aca.4.e64713
 04-03-2021
 Unique: 1319 | Total: 2061  | Access Period:
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Conference Abstract
Is cytochrome c oxidase subunit I (COI) the right DNA barcoding marker for the Chaetopteryx villosa group?
    Dalila Destanović, Lejla Ušanović, Lejla Lasić, Jasna Hanjalić, Belma Kalamujić Stroil
10.3897/aca.4.e64707
 04-03-2021
 Unique: 1115 | Total: 1805  | Access Period:
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Conference Abstract
Assessing accuracy and completeness of GenBank for eDNA metabarcoding: towards a reliable marine fish reference database
Cristina Claver, Oriol Canals, Naiara Rodriguez-Ezpeleta
10.3897/aca.4.e64671
 04-03-2021
 Unique: 1407 | Total: 2157  | Access Period:
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